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建立了大鼠尿样中调环酸钙的高效液相色谱(HPLC)测定方法。样品经Waters OasisMAX 3cc固相萃取小柱净化,Poroshell 120EC-C18色谱柱(100×4.6 mm,2.7μm)分离,甲醇-0.01%甲酸水溶液梯度洗脱,流速为0.4mL/min,检测波长275nm。研究结果表明,尿样中调环酸钙的质量浓度在0.205.00μg/mL范围内线性关系良好(R2=0.999),检出限为0.06μg/mL,定量限为0.19μg/mL,回收率在96.41%~104.94%之间。日内相对标准偏差(RSD)为2.14%3.89%,日间RSD为5.01%7.58%。该方法前处理简单可行,适合尿样中调环酸钙的测定。
Abstract：Prohexadion-calcium was purified by Waters OasisMAX column(3 cc)cartridges and determinated by HPLC-UV.The standard curves for prohexadion-calcium showed good linearity in the range of 0.20-5.00μg/mL with the correlation coefficient R2=0.999.The recoveries were 96.41%-104.94%.The relative standard deviations(RSDs)of the interassys were 2.14%-3.89% and RSDs of intraassays were 5.01%-7.58%,respectively.The detection limit of prohexadion-calcium was 0.06μg/mL,and the limit of quantification for prohexadion-calcium was 0.19μg/mL.This method was simple and suitable for the determination of prohexadion-calcium in urine.
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基本信息:
DOI：10.13526/j.issn.1006-6144.2018.02.030
中图分类号:O657.72;R99
引用信息:
[1]马安萍,陈红红,阮小林,等.固相萃取-高效液相色谱法测定大鼠尿样中调环酸钙[J].分析科学学报,2018,34(02):291-293.DOI:10.13526/j.issn.1006-6144.2018.02.030.
2018-04-17
2018-04-17
2018-04-17