| 27 | 0 | 21 |
| 下载次数 | 被引频次 | 阅读次数 |
线粒体作为能量代谢和凋亡调控的关键亚细胞器之一，其黏度的异常变化与多种疾病密切相关。而现有线粒体黏度荧光探针普遍依赖线粒体膜电位(MMP),严重抑制其在病理条件下的应用。为解决上述问题，以吩噻嗪和噻吩基团作为供给电子基(D),乙烯基为π桥，吲哚衍生物作为受电子基(A),构建了一种D-π-A构型的新型荧光探针ZXY。利用紫外-可见吸收光谱和荧光发射光谱对探针的光物理性质进行研究。结果证实，探针ZXY具有优异的黏度响应性和选择性。共聚焦成像实验显示，探针ZXY靶向在线粒体部位不依赖于MMP,且对细胞内黏度变化监测具有高度灵敏性。
Abstract：Mitochondria, as one of the key organelles involved in energy metabolism and apoptosis regulation, has abnormal viscosity changes closely associated with various diseases.However, existing mitochondrial viscosity fluorescent probes generally rely on mitochondrial membrane potential(MMP),severely limiting their application under pathological conditions.To address this issue, this study constructed a novel fluorescent probe ZXY with a D-π-A configuration using phenothiazine and thiophene groups as electron donors(D),vinyl as the π-bridge, and indole derivatives as electron acceptors(A).The photophysical properties of ZXY were studied using UV-Vis absorption and fluorescence emission spectra.The results confirmed that probe ZXY exhibited excellent viscosity responsiveness and selectivity.Confocal imaging experiments revealed that probe ZXY specifically localized to the mitochondrial region independent of mitochondrial membrane potential(MMP) and exhibited high sensitivity to alterations in intracellular viscosity.
[1] Pan X,Zhao Y,Wang J L,et al.Journal of Materials Chemistry B,2025,13(1):177.
[2] Wang L C,Song W Q,Li F X,et al.Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2026,346:126847.
[3] Yuan L C,Liu Y T,Guan C Z,et al.Talanta,2025,285:127364.
[4] PENG H.Construction and Biological Properties of a Novel Near Infrared Viscosity Fluorescent Probes:Nanning:Guangxi University(彭慧.新型近红外粘度荧光探针的构建及生物性能研究.南宁：广西大学),2024.
[5] DAI L X.Design,Synthesis and Bioimaging Applications of MitochondriaL-targeted Molecular Rotor Viscosity Fluorescent Probe.Jinan:Jinan University(代礼旋.转子型线粒体靶向粘度荧光探针的设计、合成及生物应用研究.济南：济南大学),2021.
[6] Zhang R Y,Niu G L,LI X C,et al.Chemical Science,2019,10:1994.
[7] Lu X L,Zhan Y,He W.Journal of Photochemistry and Photobiology B:Biology,2022,234:112528.
[8] LIU Q L.Synthesis and Properties of Fluorescent Probes Based on Phenothiazine Derivatives for the Detection of Hypochlorous Acid and Sulfur Dioxide.Nanchang:Jiangxi Science and Technology Normal University(刘倩玲.基于吩噻嗪衍生物检测次氯酸和二氧化硫荧光探针的合成及性质研究.南昌：江西科技师范大学),2023.
[9] Wang Z M,Liu Q C,Cai S T,et al.Dyes and Pigments,2022,203:110382.
[10] Tan X D,Hong J X,Jiang X Y,et al.Analytical Chemistry,2024,96(37):14860.
[11] GUO D Y.Construction and Application of Fluorescent probes for Monitoring Mitochondrial Membrane Potential and Autophagy.Jinan:Jinan University(郭丁一.用于检测线粒体膜电位和自噬过程荧光探针的构建及其应用的研究.济南：济南大学),2021.
[12] Wu X,Fu G Y,Li Y,et al.Analytical Chemistry,2023,95(6):3544.
[13] Fu W J,Wei W,Shen J C,et al.Chemical Engineering Journal,2025,517:164350.
[14] Wang X Y,Chen Y Y,Liu C J,et al.Analytical Chemistry,2025,97(19):10244.
[15] ZHANG T.Design,Synthesis and Biodetection Performance of Fluorescent Probes Based on Coumarin and Rhodamine Scaffords.Zhengzhou:Henan University(张恬.基于香豆素和罗丹明母核的荧光探针设计、合成及生物检测性能研究.郑州：河南大学),2024.
[16] ZHOU J L.Design of Near-Infrared Tetraphenylethene Viscosity Fluorescent Probe:Application to Transgultaminase-2 and Its Role in Tumor Cell Microenviroment.Xi’an:Xidian University(周佳林.近红外高灵敏粘度荧光探针的设计及其对转谷氨酰胺酶-2与肿瘤细胞微环境关系的研究.西安：西安电子科技大学),2021.
[17] BIAN Y Y.Synthesis and Performance Analysis of Fluorescent Probes for Detecting Viscosity and Bioactive Species.Tianjin:Tianjin University of Technology(边雅烨.检测粘度及生物活性物种荧光探针的合成及性能研究.天津：天津理工大学),2022.
[18] DU Y T,PAN C X.Journal of Yunnan University(Natural Sciences Edition)(杜宇婷，潘彩霞.云南大学学报(自然科学版)),2023,45(4):920.
[19] YU A,FENG Y,WU Z Y,et al.Fine Chemicals(余奥，冯艳，武紫燕等.精细化工),2024,41(8):1693.
[20] Huang Y F,Gao Z F,Fan Z F,et al.Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2025,342:126488.
基本信息:
DOI：10.13526/j.issn.1006-6144.2025.07026
中图分类号:Q503;O657.3
引用信息:
[1]赵欣悦,黄程,吴运军,等.一种不依赖于膜电位的线粒体黏度荧光探针的合成及性质研究[J].分析科学学报,2026,42(03):258-264.DOI:10.13526/j.issn.1006-6144.2025.07026.
基金信息:
安徽省高校自然科学研究项目(2023AH051762); 国家级大学生创新创业训练计划项目(202510368048); 安徽省大学生创新创业训练计划项目(S202510368012)
2026-05-28
2026-05-28
2026-05-28