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建立了超高效液相色谱-串联质谱(UPLC-MS/MS)检测化妆品还原液中4-氨基联苯的方法。化妆品试样经过连二亚硫酸钠化学还原后,离心、PCX固相萃取小柱提取富集、净化,并经氨化甲醇洗脱、氮吹至近干,定容后通过UPLC-MS/MS仪检测,分离柱为Waters Acquity BEH C18柱;流动相为0.3%乙腈-甲酸水溶液;流速0.5mL/min。5种不同基质中4-氨基联苯在5～250ng/mL浓度范围内呈良好的线性关系,线性回归系数r2均大于0.999,检测限均为1ng/mL。5种不同基质中4-氨基联苯的回收率为80.9%～92.6%,相对标准偏差(RSD)为0.51%～3.76%(n=3)。
Abstract：A simple and rapid method was developed for the determination of 4-aminodiphenyl reduced from azo dyes in cosmetics by ultra performance liquid chromatography-tandem mass spectrometric( UPLC-MS /M S) method.After chemically reduced with sodium dithionite, the cosmetic sample was centrifuged,e xtracted, purified by PCX solid-phase extraction column, eluted with ammonia methanol and dried under nitrogen.After the chromatographic separation on Waters Acquity BEH C18column(1 00× 2.1 mm, 1.7μ m) by 0.3% formicacid-acetonitrileisocratic elution, the analysis was carried out by UPLC-MS /MS.The linear ranges of 4-aminobiphenyl in five cosmetic matrices were 5-250ng /m L( r 2 > 0.999) and the detection limits were 1ng /mL.The recoveries were ranged from 80.9%to 92.6%, and the relative standard deviations were 0.51%-3.76%( n=3).
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基本信息:
中图分类号:O657.63;TQ658
引用信息:
[1]雷云,林雅静,戴明,等.超高效液相色谱-串联质谱法测定化妆品还原液内的4-氨基联苯[J].分析科学学报,2012,28(06):831-834.
基金信息:
福建省自然科学基金(No.2010J05019); 福建省卫生厅基金(2010-1-34)
2012-12-20
2012-12-20